Part:BBa_K2583003:Design

GPA1_modify

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 585
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2146
  Illegal XhoI site found at 1954
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 515
  Illegal BsaI site found at 1482
  Illegal SapI.rc site found at 1329
  

Design Notes

Replace C terminal 5'AAAATTGGTATTATA 3' of S.cerevisiae GPA1 with human Gαi1 5' GATTGTGGTCTCTTTTAA 3' The EcoRI restriction enzyme site is mutated.

Source

Saccharomyces cerevisiae S288C Chromosome: VIII; NC_001140.6 (113499..114917, complement) sequece found through GeneBank https://www.ncbi.nlm.nih.gov/gene/856394